[quote=Kaga]
Well that depends on which manga.If you're talking about the original manga series, then that would actually be episode 5, I believe. Things get dark at the anime equivalent of the Trigun Maximum manga series, which is episode 12. But yeah, that is what I was trying to get to. The first half of the anime is mostly jokes with some serious moments - the second half of the series is the exact opposite, with mostly with only a few scattered moments of humor.
[/quote]

Yeah, like when one of Knive's henchman kill [REDACTED] making what'sherface (It's been awhile) Sad and trigger-happy.