Yeah, Botan accepts both Shuichi and Yoko, she's not about to pretend they're the same because she knows they're different but also rather connected, even when Yoko could now survive without his human companion. Botan isn't overly gushy, jealous, irrational or rude. Sure, she gets a little out of control in rare instances but that's because she's pretty loyal to her friends and refuses to let harm come to them. And she also has a flirty/playful/dangerous side as well. If Yoko did try to harm Botan to test Shuichi, it would be pretty sweet to see the boy refuse it to happen. Both guys need to be able to protect Botan since she doesn't have the same power as Kurama, but at the same time they'd probably be able to back off and let her have at a demon when she's learned her basic fighting (thanks to Kurama on the roof during lunch). 

Getting Hiei's approval would be pretty big for Hiei since he's been pretty much "not a fan" of her for pretty much the entire series (why, I dunno). But then again, I don't see Hiei being too prominent in the human world anyway, he'd probably patrol the demon world and only see Kurama every so often. But I could see Koenma trying to butt in and say he's got a new case for the happy couple. He'd happen to bring a spray bottle with him, but I don't think Botan nor Kurama would enjoy it. And hey, you don't wanna get between a fox and his treasure now do you? Not one like Yoko anyway, him being pretty protective of their alone time would be pretty awesome.

I think Botan would be pretty happy to let the kids do their own thing though. She'd be the proud parent no matter what. I think she has a love for human life but if her kids decide to live in another realm, she would know they have allies to help them out (as well as enemies too). So I don't see it being hard for her to let go, considering she's seen Yusuke and the others grow up, she's already shed plenty of tears of joy and of annoyance. XD 

EDIT - We better stop hypothesizing though because now I just wanna RP it lol